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    <title>Nature Network - model organisms</title>
    <description>The latest taggings for model organisms</description>
    <link>http://network.nature.com/announcements</link>
    <language>en-us</language>
    <ttl>40</ttl>
    <item>
      <title>filamentous fungi transformation with  amds selective marker</title>
      <description>
        <![CDATA[Dorothy Clyde - I am doing Trichoderma reesei transformation with amds seletive marker. For preparation of selective medium,10mM acetamide as the sole nitrogen source,Cscl was addded to reduce the growth of background,agarose was used to replace of agar.But however on the 3th day,the]]>
      </description>
    </item>
    <item>
      <title>yeast isolation</title>
      <description>
        <![CDATA[Dorothy Clyde - hello friends my topic for project is production of alcohol and poly alcohol from agricultural n industrail waste using yeast.so my first job is to isolate yeast from various source.well i started isolating yeast from various fruits by using gye]]>
      </description>
    </item>
    <item>
      <title>protein expression in bacteria</title>
      <description>
        <![CDATA[Dorothy Clyde - Hello, I need to produce a protein in bacteria and have no experience in that field. I need the protein to produce an antibody against it. I've cloned its coding sequence in the mammalian vector pcDNA3. This vector has the]]>
      </description>
    </item>
    <item>
      <title>Neuronal cell suspension from Mouse brain</title>
      <description>
        <![CDATA[Dorothy Clyde - Dear friends, I am new to Neuroscience field, I want to prepare neuronal cell suspension from adult mouse brain, if any body have well experience in this procedure, please share the protocol. Thanking u in advance.]]>
      </description>
    </item>
    <item>
      <title>find a protocol to harvest mice peyer'a patch and draining lymph nodes from aortic arch</title>
      <description>
        <![CDATA[Dorothy Clyde - Dear all, Can I seek for help in harvesting mice peyer'a patch and draining lymph nodes from aortic arch? I am not sure how to distinguish them from surrounding connective tissue. Is there any clear picture published yet? Thanks a]]>
      </description>
    </item>
    <item>
      <title>How do I manage mites in my Drosophila stocks?</title>
      <description>
        <![CDATA[Dorothy Clyde - A question for all _Drosophila_ researchers: although our own stocks are free of mites, I have noticed that newly acquired stocks from other sources are sometimes not so healthy! What is the best method of quarantine to avoid contaminating all]]>
      </description>
    </item>
    <item>
      <title>hydrogen peroxide detection</title>
      <description>
        <![CDATA[Dorothy Clyde - does anybody know how reliable is nbt staining for h2o2 detection in arabidopsis.. also to do fluorescent staining by DCF ..how to avoivd background]]>
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