<?xml version="1.0" encoding="UTF-8"?>
<rss version="2.0" xmlns:dc="http://purl.org/dc/elements/1.1/">
  <channel>
    <title>Nature Network - method</title>
    <description>The latest taggings for method</description>
    <link>http://network.nature.com/announcements</link>
    <language>en-us</language>
    <ttl>40</ttl>
    <item>
      <title>Antibody Cross reactivity </title>
      <description>
        <![CDATA[phoenix red - have inherited some primary antibody in my lab which seems to cross react giving me tremendous background on my westerns. So…the recombinant protein was harvested from e-coli which was poorly purified resulting in e-coli protein being present in the product.]]>
      </description>
    </item>
    <item>
      <title>Neuronal cell suspension from Mouse brain</title>
      <description>
        <![CDATA[Dorothy Clyde - Dear friends, I am new to Neuroscience field, I want to prepare neuronal cell suspension from adult mouse brain, if any body have well experience in this procedure, please share the protocol. Thanking u in advance.]]>
      </description>
    </item>
    <item>
      <title>Alternative to TUNEL assay</title>
      <description>
        <![CDATA[Katharine Barnes - I have some technical problems (High background, non-specific signal...) with a TUNEL assay performed on fixed mouse retina slide. This assay worked very well on cultured cells, but not on tissue section. Did you have an alternative method (Annexine 5A]]>
      </description>
    </item>
    <item>
      <title>Nature Protocols Discussion Forum</title>
      <description>
        <![CDATA[Dorothy Clyde - For discussions of techniques – what works and what doesn’t. This site is for the posting of questions about methodological problems encountered in your research. We encourage you to both post and reply. We also welcome suggestions of forthcoming meetings]]>
      </description>
    </item>
    <item>
      <title>Specific anti-HA tag antibody.</title>
      <description>
        <![CDATA[Katharine Barnes - Hallo everybody. Does anybody know really specific anti-HA tag antibody which could be used also for immunofluorescence? I am using 3F10 from ROCHE and that's generating serious background (WB and IFM). Thanks, Marek]]>
      </description>
    </item>
    <item>
      <title>Enriching for spliceosomes for affinity chromatography</title>
      <description>
        <![CDATA[Katharine Barnes - I wish to purify spliceosomes from Caco2 cells to include the SC35 splicing speckles. I would like to use these enriched spliceosomes to see which of these would interact with my peptide of interest using affinity chromatography. Does someone have]]>
      </description>
    </item>
    <item>
      <title>Specific anti-FLAG tag antibody. </title>
      <description>
        <![CDATA[Katharine Barnes - Hello everybody. Does anyone know really specific anti-FLAG tag antibody which could be used for Western blot from various mouse tissues? I am using M2-anti-FLAG-HRP from Sigma and that’s generating multiple bands, specifically a band around 45 kDa which is]]>
      </description>
    </item>
    <item>
      <title>Nature Protocols Discussion Forum</title>
      <description>
        <![CDATA[Katharine Barnes - For discussions of techniques – what works and what doesn’t. This site is for the posting of questions about methodological problems encountered in your research. We encourage you to both post and reply. We also welcome suggestions of forthcoming meetings]]>
      </description>
    </item>
    <item>
      <title>Wanted: Methods for purifying DNA from agarose gels</title>
      <description>
        <![CDATA[Andrew Barrett - I am relatively new to molecular biology and need to purify DNA from agarose gels. At present, I am phenol:chloroform extracting DNA directly from low melting point agarose - with limited success. I am aware that I could also electro-elute]]>
      </description>
    </item>
    <item>
      <title>How do I manage mites in my Drosophila stocks?</title>
      <description>
        <![CDATA[Andrew Barrett - A question for all _Drosophila_ researchers: although our own stocks are free of mites, I have noticed that newly acquired stocks from other sources are sometimes not so healthy! What is the best method of quarantine to avoid contaminating all]]>
      </description>
    </item>
  </channel>
</rss>
