Solid-state NMR methods improvement.

Our group works on establishing proton detection in ssNMR as a regular tool for assignment and structure determination of proteins.
The general approach is a proton dilution in order to avoid dipolar coupling provoked line broadening.
Combination of the this labeling scheme with data acquisition accelerated by PRE (paramagnetic relaxation enhancement) allows for proton detected out-and-back experiments for correlation of backbone nuclei facilitating high protein structure and dynamics determination with a single sample.