Ilya Finkelstein's profile

What I do

Our cells are constantly subjected to intracellular and extracellular stresses that threaten genomic fidelity. Double strand breaks (DSB) in DNA are a form of damage that may arise spontaneously during DNA replication or as a result of external factors such as ionizing radiation or chemical damaging agents. Upon sensing DSB, the cell initiates a host of responses that identify, encapsulate, and ultimately process the damaged site. The goal of my work is to directly visualize the behavior of several key proteins that participate in DSB repair. Fluorescence and biochemical assays will probe the mechanism of protein-DNA association and observed nuclease activity of the complex in the context of DNA binding.

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Publications

  • Finkelstein I, Ishikawa H, Kim S, Massari A, Fayer M. Substrate binding and protein conformational dynamics measured by 2D-IR vibrational echo spectroscopy. Proceedings of the National Academy of Sciences of the United States of America 104 (8) , 2637-42 (2007) (Epub 12 Feb 2007) PubMed ID:(17296942 )

  • Finkelstein I, Massari A, Fayer M. Viscosity-dependent protein dynamics. Biophysical journal 92 (10) , 3652-62 (2007) PubMed ID:(17446536 )

  • Finkelstein I, Zheng J, Ishikawa H, Kim S, Kwak K, Fayer M. Probing dynamics of complex molecular systems with ultrafast 2D IR vibrational echo spectroscopy. Physical chemistry chemical physics : PCCP 9 (13) , 1533-49 (2007) (Epub 20 Feb 2007) PubMed ID:(17429547 )

  • Ishikawa H, Finkelstein I, Kim S, Kwak K, Chung J, Wakasugi K, Massari A, Fayer M. Neuroglobin dynamics observed with ultrafast 2D-IR vibrational echo spectroscopy. Proceedings of the National Academy of Sciences of the United States of America 104 (41) , 16116-21 (2007) (Epub 04 Oct 2007) PubMed ID:(17916624 )

  • Kwak K, Park S, Finkelstein I, Fayer M. Frequency-frequency correlation functions and apodization in two-dimensional infrared vibrational echo spectroscopy: a new approach. The Journal of chemical physics 127 (12) , 124503 (2007) PubMed ID:(17902917 )

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