Brian Reed's profile

What I do

A little peptide biotransformation mass spectrometry, a little chromatin immunoprecipitation/histone modification studies from rat brain, a little cocaine and heroin abuse research, a little real-time optical PCR, a little microdialysis, the occasional radioimmunoassay for peptide quantification and high-performance liquid chromatography-uv vis for drug abuse metabolite quantificaition.

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Interests

My interests have evolved; my training in graduate school was in analytical biochemistry, with an emphasis on protein purification, mass spectrometry, HPLC, and electrochemical detection. I am currently doing research on drug abuse in a laboratory that spans the gamut from molecules to drug addicted patients, with various cell-culture and animal models in between. I still get a great deal of satisfaction in the development of novel techniques, but now find much mor einterest in thinking about the neurobiology and neurochemistry of drug addiction, and how the findings in model organisms translate to actual human patients.

Projects

Neuropeptide Biotransformation – Mass spectrometric studies in brain, plasma, and cerebrospinal fluid, with an emphasis on the endogenous opioid peptides.

Alterations in chromatin structure at the promoters of opioidergic and other stress-related neuropeptidergic genes in response to cocaine and heroin

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