• Ronnie Wei's profile

    Boston

    • What I do

      I am carrying out biochemical and structural analysis of kinetochore complexes in mediating chromosome-microtubule (MT) attachment. The kinetochore function is crucial for the faithful transmission of genetic informaion through each and every cell division. Aberrations in this process have been associated with many types of cancer and human genetic disorders. The goal is to unravel the machanism governinging the dynamic interaction between chromosome and mitotic spindle and how this cellular events affect cell proliferation. Many of the cellular machineries mediating and regulating chromosome segregation are emgering to be novel cancer therapy targets, incuding Hec1, Aurora B kinase, which are the main focus of my research. My approach encompasses a range of structural techniques coupled with functional assays to demonstrate that Hec1/Ndc80 forms a direct kinetochore-microtubule interface and this interface is regulated by Aurora B phosphorylation.

    • Affiliations

      Current

    • Interests

      cell cycle control, protein chemistry, protein expression and characterization, transcriptional regulation, protein crystallography and NMR spectrometry.

    • Projects

      Biochemical and structural analysis of kinetochore complexes in mediating chromosome-microtubule (MT) attachment. Study of the Aurora B kinase in the mitotic checkpoint regulation. *Biochemical characterization of protein-RNA interactions.

    • Publications

      • Structural analysis of Bub3 interactions in the mitotic spindle checkpoint Proc. Natl. Acad. Sc.i U S A. 104 , 1201-1206 (2007)

      • The Ndc80/Hec1 complex is a contact point for kinetochore-microtubule attachment Nat Struct Mol Biol. 14 , 54-59 (2007)

      • Crystal and NMR studies of the kinetochore Spc25/Spc24 globular domain Structure 14 , 1003-1009 (2006)

      • Molecular organization of the Ndc80 complex, an essential kinetochore component Proc. Natl. Acad. Sc.i U S A. 102 , 5363-5367 (2005)

      • Identification of an RNA-binding Site in the ATP-binding Domain of Escherichia coli Rho by H2O2/Fe-EDTA Cleavage Protection Studies J. Biol. Chem. 276 , 28380-2838 (2001)

      view all
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