There has been a moment of great excitement at my desk: There is a Network Protocol, based on a Nature paper, that has a query from a reader, and a reply with useful information.
Counting Marine Microbes with Guava Easy-Cyte 96 Well Plate Reading Flow Cytometer
" Wonderful and straight forward however is the SYBR green the one supplied by invitrogen? In which case is the working concentration 5x?
Thank you
Posted by: Ssemakalu Cornelius | June 8, 2009 01:15 PM
Yes, we use Sybr Green from Invitrogen. We use a 1:20 or 1:30 dilution of the stock and stain cells at a 1:100 dilution so it is a final dilution of 1:2000 or 3000. It is possible that the 1:3000 dilution gives less background noise. We stain for at least one hour before counting.
Posted by: Kevin Vergin | September 25, 2009 09:38 PM "
While this is not the first useful discussion on our site, these exchanges are sufficiently rare for it to be a cause for celebration!
Hoorah! I know how rare user comments are (so why am I building them into the new f1000 site? Beats me) so well done!
Yes, exactly! The Nature news website is the only part of the journal open for comments so far – the three years or so of experience there is mixed. Some of the conversations are so interesting, others are, er, less so!
But it is certainly a cause for celebration when any online publication, from eminent journal like Nature Protocols , to a humble little personal blog, receives a useful, informed comment/exchange. Congratulations. And good luck to you, Richard – glad to read that you are building this feature into your new site.
Thank you for the comments! (I am smiling).
It would be really great if this type of science communication started to take off…