Sequencing .... please help
Shamsher pratap sandhu
Saturday, 21 February 2009 04:26 UTC
I did PCR and then sequencing with Reverse primer for that PCR product. the seq showed forward primer seq twice (that is repetition of forward primer seq). I did not understand why it is like this. I did seq for many samples. But never did I get the problem like this. any idea?
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Replies
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Interesting..
What I can think of.. is your forward primer was double in lenght with the same sequence…
i mean.. suppose you order forward primer like.. this…
atatatatatatatatatata
But company send u..
atatatatatatatatatata+atatatatatatatatatata
this is how when u PCR.. you will get double forward primer insertion..
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I would not worry about this. Some freak results should not slow down your momentum in real research.
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