measurement accuracy for TAE buffer
Tiffiny Baring
Friday, 12 June 2009 03:47 UTC
how detrimental will it be to my gels if i tried making a stock 50X TAE buffer and ended up with around 1100 ml (or 1200?) instead of 1000 ml? so i know it’s less concentrated, do we really have to adjust measurements every time we want to make a 1X working solution? or should i just find a job at starbucks instead?
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Replies
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I would suggest you post your question in the Nature Protocols forum.
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It does not matter for DNA Gel. You can use the 0.5 x TAE running buffer.
So dont worry about that. -
i am also using TAE Buffer.some times we differ the concentration fom 1x but we are getting same results.slight differ are adjusttable. so need not to worries about that.
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thank you very much for all your input!
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