Welcome to the biomolecular NMR forum!
Michael Durney
Thursday, 15 February 2007 19:49 UTC
When the forum is active we will discuss topics such as:
The evolving role of NMR in drug discovery and development;
Methodology to produce “difficult” samples that may not crystallize (eg: cell-free, protein-RNA, membrane proteins);
A rigorous appraisal of software for assignment and structure calculation;
A “journal club” where members can post a short critique on interesting papers for discussion.
Participants can of course suggest new topics! Structural biology is a very exciting field especially in the context of mechanistic studies of biomolecular function by molecular and cell biology techniques informed by X-ray crystallography and cryo-EM in addition to NMR. Hopefully this forum will be of some use in identifying and discussing interesting problems where NMR can make a unique and complementary contribution.
Please invite anyone you know who might be interested to join the group.
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Replies
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Hi Everyone
I am wondering if anyone can help me out.
I have been encountering a problem with my structure determination. The NMR of some of the compounds have broad carbon signals as well as broad proton signals. This makes it difficult to run a 2-D experiment such HMBC with reasonable result. Can anyone please suggest a way around it? I am dealing with natural products from plants.
Thank you. -
Hi kenneth, maybe the pressence of certain paramagnetic atoms cause the line bradening, are adding EDTA to your samples, 50 uM can solve your problems. Other reason can be the slow correlation time (Tc) due to high concentration of salts or other components in your buffer. Obviously these are not due to the big MW of your target? Best Regards
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