Hi everyone! I’m working with a intregal membrane protein over-expresed in human cell lines. My principal problem is to find the optimal conditions for solubilization, I know that my protein is very unstable. Recently I read the paper from Ericcson and colleages about the thermofluor Assay. Despite the low sensibility due to the detergents I need to solubilizate my protein I think the assay will work correctly if I can configure the thermal cicler. I’m using the Bio-Rad MyiQ real time PCR system, but I can’t configure it to correctly read Sypro Orange fluorescence, Anyone has used it? Thanks