Our lab has spent many years purifying rabbit polyclonal anti-peptide antibodies from serum with good yield using Reacti-Gel HW-65F from Pierce.
These beads have a particle size of 32-63um and were used in affinity columns that were made by reacting the beads with the peptide of interest passing the anti-sera through the column then eluting the bound antibody with 100mM glycine buffer pH2.2
The problem is we can no longer buy this product. It is no longer supplied by Pierce and we cannot find an alternative supplier.
The replacements sent by Pierce have been Reacti-Gel(6x) Support and Reacti-Gel (GF-2000) neither of which have given very satisfactory results.
The yield is very low and with some antibodies no purification is possible at all. I have also tried the Pierce AminoLink Kit with ready made columns and reagents but none compare to our original Reacti-Gel.
If any-one has had similar problems or can offer any suggestions or advice or information as to where we may locate our original beads or something similar I would love to hear from you.