Hi, I’ve recently started woking with Tricine gels. I’m looking to resolve pepetide in the 1-10 KDa range. The Schagger staining protocol suggests incubating gel in fixing sol (50% MeOH, 10% acetic acid and 100 mM ammonium acetate prior to staining with 0.025% coomassie in 10% acetic acid. My problem is that my 1mm,16% tricine gel bloats up after the above step and protein bands appear very diffused. Also, I’m having problems while trying to lift the gel from the plate while dismantling. What can I do to rectify this?

Thank you for yr response!