Tricine SDS-PAGE
Claire Jennings
Monday, 01 June 2009 22:02 UTC
I’ve recently tried running tricine SDS-PAGE gels using the protocol described by Schagger (2006). It’s a great paper but after setting up and running the gels on several times, I keep finding that my protein samples can migrate through the 4% stacking gel but not into the 16% acrylamide resolving gel. I have re-checked my acrylamide concentrations and powerpack conditions. Any suggestions on how I might solve this problem would be much appreciated.
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u can also add butanol after adding resloving gel…….. after it is solidified rinse it properly to remove it completly……..then cast stacking..
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