request for transcription factor ELISA protocol
Annemieke ten Bokum
Wednesday, 13 May 2009 14:50 UTC
Hi all,
I need a validated method for binding oligonucleotides to ELISA plates so I can study interaction of promoter sequences with a transcriptional repressor. We have a working EMSA so I have binding conditions etc., I just need something that is more quantitative, more sensitive, and more high throughput. I also have the recombinant protein, His-tagged.
I am grateful for any literature references to methods papers.
Thanks in advance,
Annemieke ten Bokum
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