polyacrylamide gel
Amit Kumar
Saturday, 18 April 2009 00:02 UTC
I am using 18 % SDS-PAGE gels for my 4.5 kDa amyloid protein…but every time I run a gel I see a big smear instead of discrete bands at the end of the gel….can anyone tell why this is happening…is something wrong with my ingredients.
Second I also see a band Patern around 50-60 kDa range I have check it with western blot and it is not protein …..what it is some kind of contamination…
Please Help
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Hi Xionghui,
My recombinant protein is without a fusion tag…but my protein has a very high aggregation propensity
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I see, I guess your protein is highly hydrophobic, I am thinking maybe you can include some detergent in the TEV buffer, such as 0.1% triton or NP40, hope that help.
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yes my protein is highly hydrophobic…..have you ever have such kind of problem xionghui
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yes,I have been with some hydrophobic protein, but not with the problem of protease cleavage.
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