IgG purification
nishanth nataraj
Saturday, 04 August 2007 14:18 UTC
can any one tell me why v use ammonium sulhate for precipitating IgG molecule not for IgM and why not with other salts
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Replies
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ammonium sulphate is highly soluble in water and competes with protein to form hydrogen bonds with water.
different proteins have different number of hydrogen bonding interactions with water depending on charge or polarity of residues exposed on the surface of a particular protein. so one can precipitate different proteins with different concentrations of ammonium sulphate.
this method is not limited to igG or igM.
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