Request for a protocol for extracting RNA from cells in agar.
Dorothy Clyde
Wednesday, 27 June 2007 11:53 UTC
From the moderators: The following request was originally posted anonymously as a reply in the Cancer Stem Cell thread of this forum. We have re-posted it here, as a new topic, to increase its visibility and to encourage responses.
Great Forum!!! I was wondering if anyone has tried extracting RNA from cells in Agar. I was growing some cells to look at the colonogenicity and wanted to look at the message level also but I cannot find a way to extract RNA. Qiagen tech support was not able to help. If anyone has any suggestions please let me know!!!
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Replies
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I would suggest using the Bio 101 “FastPrep” method: It’s an extraction kit that uses a small tube half filled with small beads which is shaken vigorously in a specialized machine (known as a bead-beader or “FastPrep machine”). The tube contains chemicals that stabilized the DNA/RNA as it is released from the lysed cells.
I haven’t worked with cells in agar my-self, but I have successfully used this method for extracting DNA (an RNA kit is also available) from really difficult sources like raw soil samples.
Link: http://www.qbiogene.com/fastprep/
(It appears that Bio 101 is now part of Q-Bio Gene).I hope this can be used
-Rasmus Wernersson
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