scFv expression
Sun Yi
Sunday, 24 August 2008 15:48 UTC
I’ve selected the phage display antibody(scFv) library and transduced the phagemid into HB2151 for making scFv. But I can’t get the scFv in several conditions, does any one know the optimal expression condition(especially the expression system,the concentration of glucose and IPTG, the tempreture,ect)which was determined empirically?
Thanks in advance!
Updated 25 August 2008 05:23 UTC
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