Specific anti-FLAG tag antibody.
Mary Zhang
Monday, 28 May 2007 09:03 UTC
Hello everybody.
Does anyone know really specific anti-FLAG tag antibody which could be used for Western blot from various mouse tissues? I am using M2-anti-FLAG-HRP from Sigma and that’s generating multiple bands, specifically a band around 45 kDa which is unfortunately my expected band located?
Thanks,
Mary
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Replies
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Hi Mary,
M2 anti-FLAG tag antibody is usually very good for WB. Try to dilute it much more. What conditions do you use? Also, pre-adsorbtion of the diluted antibody on the old blotted membrane helps.
Good luck,
Marek -
Hi,
Flag tag gives a lot of background especially in brain tissue. The problem is not in the antibody but that the FLAg sequence is present naturally resulting in this background.
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Dear Marek,
Sorry for replying too late. Many thanks. I’ll try according to your suggestion.
Cheers,
Mary
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