Hi
I am working on Na/K ATPase detection in kidney tissue by western blotting. Using the antibody specific for alpha-1 subunit, I am getting 2 distinct bands- one around 100 kDa and the other around 50 kDa. The 100 kDa one is where alpha subunit is supposed to be, but I am not sure how to interpret the second band. I even tried using 2 different methods of sample preparation (boiled vs unboiled) but the bands are pretty consistent.
I would appreciate suggestions on this topic.