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• Welcome to the Nature Protocols Discussion Forum. We invite you to post questions relating to techniques and to answer the queries of others.(Locked)

  Posted by:

  Katharine Barnes

  Tags:

  • nature protocols
  • method
  • protocol
  • methods
  • protocols
  • technique
  • techniques
  • mirna
  • sirna
  • glycation
  • of
  • protein

  0 replies

• Problems with affinity purification of anti-peptide rabbit polyclonal antibodies using Pierce Resins

  Posted by:

  Edith Blackburn

  Tags:

  • hw65f
  • problems affinity columns
  • method
  • protocol
  • technique
  • antibody
  • protein
  • purification
  • biochemistry and protein analysis
  • immunological techniques
  • isolation purification and separation

  2 replies

  last reply:
  29 November 2007 by Edith Blackburn

• How do I manage mites in my Drosophila stocks?

  Posted by:

  Andrew Barrett

  Tags:

  • fly stock
  • drosophila
  • mites
  • quarantine
  • method
  • protocol
  • model organisms

  1 reply

  last reply:
  24 April 2007 by Simon Bullock

• Wanted: Methods for purifying DNA from agarose gels

  Posted by:

  Andrew Barrett

  Tags:

  • gel extraction
  • dna
  • cloning
  • purifying
  • agarose
  • protocol
  • method
  • isolation purification and separation
  • nucleic acid based molecular biology

  10 replies

  last reply:
  05 September 2007 by Mingyong Xiong

• Specific anti-FLAG tag antibody.

  Posted by:

  Mary Zhang

  Tags:

  • antibody
  • western
  • blot
  • protocol
  • method
  • antiflag
  • biochemistry and protein analysis
  • immunological techniques

  3 replies

  last reply:
  26 June 2007 by Mary Zhang

• Enriching for spliceosomes for affinity chromatography

  Posted by:

  Carol Trim

  Tags:

  • purify
  • spliceosomes
  • affinity
  • chromatography
  • enrich
  • protocol
  • method
  • splicing speckles
  • sc35
  • isolation purification and separation
  • biochemistry and protein analysis

  1 reply

  last reply:
  22 June 2007 by Hannah Clark

• Specific anti-HA tag antibody.

  Posted by:

  Marek Cebecauer

  Tags:

  • ha tag
  • antibody
  • immunofluorescence
  • protocol
  • method
  • biochemistry and protein analysis
  • imaging
  • immunological techniques

  4 replies

  last reply:
  21 August 2007 by Dorothy Clyde

• CFSE staining

  Posted by:

  Aziz Chentoufi

  Tags:

  • cfse
  • facs
  • protocol
  • cytometry
  • pbmc
  • isolation purification and separation
  • immunological techniques

  5 replies

  last reply:
  21 April 2009 by Christopher Dyer

• bone tumors problems

  Posted by:

  Leonardo Leonardi

  Tags:

  • tumors
  • in situ hybridisation
  • ish
  • protocol
  • staining problems
  • imaging
  • immunological techniques
  • cell and developmental biology

  0 replies

• Alternative to TUNEL assay

  Posted by:

  Raphael Roduit

  Tags:

  • apoptosis
  • tunel
  • method
  • protocol
  • cell and developmental biology
  • cell death
  • annexin
  • imaging

  2 replies

  last reply:
  08 January 2008 by Raphael Roduit

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