Molecular analysis of coding gene
nadia nouir
Wednesday, 19 September 2007 21:11 UTC
I have recently characterized a new protein with interest in serodiagnosis and I want to determine if one or more genes encode for this protein and if there are introns involved in the gene.
could somebody give me an idea how to proceed and if there are a determined protocol for that.
Is there any internet program which I can use for alignement between cDNA and genomic DNA
Thank you
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Replies
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Hello.
Supposing you know the protein sequence you can go to NCBI or Ensembl and make a blast search in the genome of interest. This should give you the gene or genes that code for it, and also other proteins that might have similar domains. In Ensembl you’ll get a nice page about that gene, with information about the protein, the messenger RNA, introns, the possibility of multiple transcripts existing, etc.
This should get you started…and there are plenty of bioinformatics tools on the internet.
You’ll find many listed here:
http://bioinformatics.igc.gulbenkian.pt/tools.phpGood luck.
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