help on cloning strategy
Meetali Singh
Monday, 13 October 2008 17:44 UTC
If we have to clone a gene which has a high G+C content relative to the bacterial host (gram -ve, maybe E. coli), then wht special strategies we need for cloning this gene?
I have recently started studying mol bio and i am not able to find some material which exactly addresses my querry
the gene can be any, if some one cn explain me in general, i will be really gratefull.
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Replies
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You can simply use the iPCR methods to clone your gene.
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you can use hotstartaq polymerase and Q-solution from Qiagen to amplify GC rich DNA fragments
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