Replies

• 1

  Tarun Gupta

  31 May 2008 | 07:13

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  In our Lab, we work on Cancer cell lines. Usually we use G418 based cytotoxic stress.

• 2

  Luca Massimino

  31 May 2008 | 07:36

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  Dear Tarun, thanks for your reply.
  Maybe I should have been more clear. I work with Multiple Myeloma cells and I need to find out newer ways than antibiotics in order to induce cytotoxicity. The problem is my ignorance in molecular biology protocols because I am formed as a Neuroscientist! =(

  For example I would like to induce:
  - Oxidative stress
  - Mitochondrial stress
  - ER stress
  - Lysosomal stress

  How can I?!?

• 3

  Tarun Gupta

  31 May 2008 | 10:17

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  Hi Luca,

  Well, simvastatin can be used to induce mitochondrial stress by inducing caspase pathway. I came across a paper that reported synergistic induction of cell death by the combination of simvastatin and doxorubicin. (Check this: Br J Pharmacol. 2004 November; 143(6): 715–724)

  Kind Regards,
  Tarun

• 4

  Luca Massimino

  31 May 2008 | 13:45

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  Well, THANK YOU!
  It looks like it induces apoptosis by activation of mitochondrial caspase 9 in lymphoma and myeloma cells: that’s perfect.
  Now I just have to look for the others stressors…

• 5

  mansooreh esmaili

  03 August 2009 | 09:13

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  HI

  oxidative stress coude be applied by using H2O2 in certain concentration .
  if you search yhe web you can find the exact concentration .
  good luck