We just published a paper that demonstrates the feasibility of in-cell NMR measurements for dissecting endogenous phosphorylation events in live oocyte cells and in cell-free extracts (as well as in in vitro reconstituted kinase reactions). I (obviously) think it’s quite a neat bit of work, especially when you are into kinase mechanisms etc..

I would love to hear your comments and about what part of the paper you like most. My personal favorite is the experiment described in supplementary figure 3. Although probably a bit difficult to grasp, I find its outcome way cool.