Replies

• 1

  Anna Kushnir

  17 October 2008 | 19:06

  [ Permalink ]

  Hi Vidhi,
  I have reposted your question in the Nature Protocols Forum. I think it is more likely to be answered there, so check that link for responses. Hope you get the help you need with your experiment! Best of luck.

• 2

  Ashish Kumar

  18 October 2008 | 00:19

  [ Permalink ]

  Hi Vidhi,
  KDR (VEGFR-2) is expressed predominantly in endothelial cells. Here is a paper that deals with lot of endothelial cell lines including MS1:

  http://atvb.ahajournals.org/cgi/content/abstract/23/11/2041?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=baec&searchid=1&FIRSTINDEX=30&resourcetype=HWFIG

  Useful excerpt:
  “Human umbilical vein endothelial cells (HUVECs) (Clonetics) and coronary artery endothelial cells (HCAECs) (Clonetics) were cultured in EGM-2 MV medium. Bovine aortic endothelial cells (BAECs) (Clonetics), human embryonic kidney (HEK)-293 cells (ATCC CRL-1573), COS-7 (ATCC CRL-1651), and mouse pancreas microvascular endothelial cells (MS1) (ATCC CRL-2279) were cultured in DMEM supplemented with 10% heat-inactivated FBS. Stable transfection of MEEP cells and transient transfection of HUVECs, HCAECs, BAECs, and MS1 cells were performed as described previously.”

  Ashish

• 3

  vidhi desai

  18 October 2008 | 03:03

  [ Permalink ]

  Thanks a lot Anna and Ashish for replying