Advances in nucleic acid detection and quantification forum: topic
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Time for a new approach?
Simon Hughes
Monday, 14 July 2008 14:56 UTC
PCR has been an essential tool in my molecular biology toolkit ever since I started working in the laboratory 12 years ago. I have taken advantage of many of the advances that have enabled improved fidelity, yield and speed of amplification, to address a wide variety of challenges. However, despite its utility, has PCR gone as far as it can? Is it time to look for more specific approaches to specific questions, rather than a “one size fits all” approach?
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Replies
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Advances in nucleic acid techniques have led to the development of more sensitive, stringent and rapid testing procedures based on nucleic acid analysis. The introduction of specific amplification techniques have made a major impact on the way in which nucleic acid analysis is carried out. One such amplification technique is NASBA (Nucleic Acid Sequence Based Amplification).
NASBA is a one step isothermal process for amplifying RNA. It has proven to be successful in detection of both viral and bacterial RNA in clinical samples. NASBA assays are gaining acceptance among the molecular biology researchers as genetic material identification technique.For more details, visit:
www.biomerieux.com
www.premierbiosoft.com/tech_notes/NASBA.html
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