one basic question?
krithika nagarajan
Sunday, 29 June 2008 10:37 UTC
generally embryonic stem cells are grown on feeder layers. the question is : when we trypsinize the embryonic stem cells, wont the feeder layer cells detach? will they contaminate our culture of ESC?
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Replies
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Hi – there’s a video of the process at:
http://www.jove.com/index/details.stp?ID=49 -
sir
thank u for responding to my query, your reply proved to be useful. I have completed M.Sc in Biomedical sciences with first class and distinction. Iam very intersted in STEM CELL research. kindly guide me to establish my careerIf you are interested I can forward my resume. this is my mail id : krithika_211@yahoo.co.in.
once again thanks a lot for responding. Iam looking forward to hear from you.
yours sincerely
N.Krithika -
The feeder cells won’t attach to the flask or other feeders bacause we inactivate before using them. This makes them non-growing cells.
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