Reply from Michael Lin

Michael Lin — Sun, 30 Mar 2008 05:22:49 -0000

Yet more tricks with GFP! What’s technically interesting about this is that it uniquely requires the self-associating character of those particular superfolder GFP fragments characterized by Cabantous and Waldo in their 2005 Nature Biotech paper. That is, high signal here requires that reconstitution be fairly easy and not depend on the fragments being held together for a long time, because the authors want proximity alone rather than molecular association to be the trigger. In contrast, detection of protein-protein interactions by BiFC/split FP requires the use of FP fragments that won’t coassociate, so superfolder GFP broken at 215 is inappropriate for that. I wonder if the enthalpy of the GFP reconstitution is big enough for it to function as a cell-cell adhesion molecule?

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Reply from Michael Lin