Recent replies to "Estrogen Receptor negative growth problems" http://network.nature.com/forums/natureprotocols/948 en-us 40 <p>You may want to split at a lower subcultivation ratio (eg 1/8 or 1/10), and then observe if they can grow to confluency. Perhaps also try pipeting up and down more when dissociating with trypsin so that they&#8217;re more evenly dispersed when dispensing into a new flask. Alternatively, try a larger flask, and see if they grow better. The following page also contains some general information on taking good care of cells: <a href="http://biologicalworld.com/cellcare.htm">Taking Care of Cells in Tissue Culture</a></p> Sun, 10 Feb 2008 03:56:54 -0000 http://network.nature.com/forums/natureprotocols/948?page=1#reply-2586 Keith Brent http://network.nature.com/forums/natureprotocols/948?page=1#reply-2586