Recent replies to "Neuronal cell suspension from Mouse brain"

Reply from Bronwen Dekker

Bronwen Dekker — Sun, 03 Feb 2008 14:18:36 -0000

There is a post on our Protocols Network that might be of use to you:

Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation

... and this one is specifically for chromaffin cells…

Rat Chromaffin cells primary cultures: Standardization and quality assessment for single-cell assays

Reply from Dorothy Clyde

Dorothy Clyde — Mon, 28 Jan 2008 15:18:18 -0000

Thanks for the link to JoVE, Graham. We think movies are a great way of explaining tricky protocols: many Nature Protocols also include movies, and we highlight them here.

I hope Kifayath finds the links useful, but maybe links to something more directly related to the requested protocol might be more useful? I’ve had a quick look through our content and unfortunately couldn’t find anything specific to preparing neuronal cell suspensions from adult mouse brains; this is clearly something we need to look into further.

Dot

Reply from Graham Steel

Graham Steel — Wed, 23 Jan 2008 11:33:47 -0000

If you do some searching here you might find something of interest.