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Alternative to TUNEL assay
Raphael Roduit
Thursday, 20 December 2007 14:02 UTC
I have some technical problems (High background, non-specific signal…) with a TUNEL assay performed on fixed mouse retina slide. This assay worked very well on cultured cells, but not on tissue section.
Did you have an alternative method (Annexine 5A staining on cryo slide?)...
Thank a lot for info
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Replies
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Been a long time since I have done it… but have you tried activated caspase 3 staining in an immunofluor reaction? It worked great for us in cryo sections… but have never tryied with mouse retina.
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Thank for the info.
Did you have a ref for the methods of staining, source of antibody???
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