Recent replies to "Problems with affinity purification of anti-peptide rabbit polyclonal antibodies using Pierce Resins" http://network.nature.com/forums/natureprotocols/64 en-us 40 <p>I have had better success recently using the new AminoLink Plus Kit from Pierce.<br />Again results not as good as the original beads but better than the other matrix offered by Pierce as alternatives.</p> Thu, 29 Nov 2007 10:06:05 -0000 http://network.nature.com/forums/natureprotocols/64?page=1#reply-1686 Edith Blackburn http://network.nature.com/forums/natureprotocols/64?page=1#reply-1686 <p>try uing CNBr activated sepharose 4B fast flow by coupling the peptide and purifing the Antibodies. If you have any better suggestion please let me know. as i am also doing the same thing but purifing the anti-peptide andtibodie fropm Rabbit is difficult by peptide affinity column.</p> Fri, 23 Nov 2007 16:10:37 -0000 http://network.nature.com/forums/natureprotocols/64?page=1#reply-1616 binny varghese http://network.nature.com/forums/natureprotocols/64?page=1#reply-1616