Recent replies to "Tags for protein purification"

Reply from Rahul Saxena

Rahul Saxena — Mon, 07 Apr 2008 20:38:38 -0000

Hi purusharth, this is rahul here…I hope u remember, well u can go to a site of Arabidopsis Biological Resource Center..They have quite a few vectors having differential tags such as MYC, HA for the bacterial expression system and that too for just 4 USD each vector..Order from their…U will definately find some of ur interest. Tk care

Reply from Abdullah Yalcin

Abdullah Yalcin — Tue, 04 Sep 2007 01:45:50 -0000

You can use S-protein tag. pET30b vector from Novagen has this tag. As far as I know, S tag and His tag are in tandem in this vector, though. But this vector has such features that allow you to rid of His or His/S tags from the protein after purification. Novagen sells S tag purification kits.

Reply from Bronwen Dekker

Bronwen Dekker — Fri, 31 Aug 2007 23:51:37 -0000

I found this website while I was looking for protein purification protocols to commission:
http://wolfson.huji.ac.il/purification/Expression_Systems.html.

Maybe it will be useful to you as well?

Best of luck!

Reply from Marek Cebecauer

Marek Cebecauer — Wed, 29 Aug 2007 11:42:03 -0000

Hi,

c-myc, GFP, HA will work in E.coli as well. HA tag usually gives higher background after purification.

Best
Marek

Reply from PURUSHARTH RAJYAGURU

PURUSHARTH RAJYAGURU — Tue, 28 Aug 2007 17:43:31 -0000

Thanks for the responses but as I have mentioned I need to use E. coli expression system and I am not sure if c-myc, HA etc would work in bacteria.
Any comments?

Reply from Frederic Guilhem

Frederic Guilhem — Mon, 27 Aug 2007 06:22:47 -0000

sorry i didn’t find how to edit my previous post.
Well HA tag exist also. The efficiency of recovery is ok, but it’s less specific. Agarose coupled anti HA is available from sigma and other brands

Reply from Frederic Guilhem

Frederic Guilhem — Mon, 27 Aug 2007 06:17:19 -0000

also GFP tag exists in mammalian cells.

Reply from Marek Cebecauer

Marek Cebecauer — Fri, 24 Aug 2007 09:34:19 -0000

Hi,

I recommend c-myc tag or even better tandem affinity purification tag. Unfortunately, I can’t provide you with any plasmids since it is a long time now I left the field.

Good luck!!
Marek