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V5 antibody for IP

huang guanrong

Wednesday, 11 Jul 2007 22:35 UTC

Hi guys,
Would anyone like to share what V5 antibody to use for IP? I use the invitrogen one, but I could not get overexpressed v5-tag protein pulled down by protein G and invitrogen mouse anti-v5.

Thanks,
Guanrong

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    • Anonymous

      20 Jul 2007 | 21:58

      Hi Guanrong,
      I used mouse anti-V5 AB from Invitrogen to IP an overexpressed V5-tagged protein with protein G beads from Amersham. I had no problems with the IP.
      Olga

    • Dorothea Peters

      07 Sep 2007 | 09:41

      Hi Olga,

      so how did you do it, which IP buffer did you use, did you leave the antibody overnight, how often did you wash the beads and with which buffer? Thanks!

      Dorothea

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