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    <title>Recent replies to "Request for a protocol for extracting RNA from cells in agar."</title>
    <description>Recent replies to "Request for a protocol for extracting RNA from cells in agar."</description>
    <link>http://network.nature.com/forums/natureprotocols/260</link>
    <language>en-us</language>
    <ttl>40</ttl>
    <item>
      <title>Reply from Rasmus Wernersson</title>
      <description>&lt;p&gt;I would suggest using the Bio 101 &amp;#8220;FastPrep&amp;#8221; method: It&amp;#8217;s an extraction kit that uses a small tube half filled with small beads which is shaken vigorously in a specialized machine (known as a bead-beader or &amp;#8220;FastPrep machine&amp;#8221;). The tube contains chemicals that stabilized the &lt;span class="caps"&gt;DNA&lt;/span&gt;/RNA as it is released from the lysed cells.&lt;/p&gt;


	&lt;p&gt;I haven&amp;#8217;t worked with cells in agar my-self, but I have successfully used this method for extracting &lt;span class="caps"&gt;DNA &lt;/span&gt;(an &lt;span class="caps"&gt;RNA&lt;/span&gt; kit is also available) from really difficult sources like raw soil samples.&lt;/p&gt;


	&lt;p&gt;Link: http://www.qbiogene.com/fastprep/&lt;br /&gt;(It appears that Bio 101 is now part of Q-Bio Gene).&lt;/p&gt;


	&lt;p&gt;I hope this can be used&lt;br /&gt;-Rasmus Wernersson&lt;/p&gt;</description>
      <pubDate>Sun, 19 Aug 2007 19:57:05 -0000</pubDate>
      <link>http://network.nature.com/forums/natureprotocols/260?page=1#reply-851</link>
      <dc:creator>Rasmus Wernersson</dc:creator>
      <guid>http://network.nature.com/forums/natureprotocols/260?page=1#reply-851</guid>
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