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IF protocol for HA anti-body

Haymanti Bhanot

Tuesday, 17 June 2008 16:17 UTC

Hi!
I have been using the HA antibody from Covance using the PFA-Gluteraldehyde procedure for Immunofluorescence. But it is giving me alot of background. Infact I have tried antibodies from Lambda, Boerhinger Mannheim and ABM as well. Could any one please tell me what protocols they follow while using an HA antibody with minimum or no background?
Thank you!