Hello,
does anybody have some advice how to increase the penetration of my anti-GFP antibody to the GFP-expressing mitochondria in the axons in the mouse spinal cord? In frozen sections. We tried different ways, such as using methanol, or mixture of methanol-chloroform…does not help…Any idea? Thanx a lot!
P.S. The Ab stains the axons, which also express GFP. So the Ab works, but it can not get into the mitos. And, I always use Triton X-100, as well