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Enriching for spliceosomes for affinity chromatography
carol McClelland
Tuesday, 08 May 2007 10:37 UTC
I wish to purify spliceosomes from Caco2 cells to include the SC35 splicing speckles. I would like to use these enriched spliceosomes to see which of these would interact with my peptide of interest using affinity chromatography. Does someone have a protocol for this process as this would be very helpful?
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Replies
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Hi Carol,
The labs of David Spector and Angus Lamond have both carried out research on various aspects of nuclear biology, including splicing speckles, and may be able to help you.
I hope this helps, and do let us know how you get on!
Hannah.
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