Recent replies to "low molecular weight peptides and spin column purification" http://network.nature.com/forums/natureprotocols/1140 en-us 40 <p>That&#8217;s really helpful, thanks for the info</p> Tue, 25 Mar 2008 13:40:58 -0000 http://network.nature.com/forums/natureprotocols/1140?page=1#reply-3270 James Ghadiali http://network.nature.com/forums/natureprotocols/1140?page=1#reply-3270 <p>Depends on from what else you are trying to separate it?</p> <p>I routinely purify a peptide of (1900Da)with Sephadex G-25 spin column (made <em>in-house</em>) Separation profile is like this</p> <p>a) Impurities of very low molecular weight (400-600 Da) and I can totally get rid-off<br />b) One of the impurities is around (~1100Da) and it always comes along with my (1900Da) product <br />c) Peptide recovery efficiency about 60-80 % if I try to increase the recovery I start observing increase in (1100Da) impurity.</p> <p>With these observation I guess you can use Sephadex G-10 for your protocol.</p> <p>Hope this help.</p> Mon, 10 Mar 2008 21:12:53 -0000 http://network.nature.com/forums/natureprotocols/1140?page=1#reply-3054 Chaitanya Saxena http://network.nature.com/forums/natureprotocols/1140?page=1#reply-3054