HI,
I am a young researcher and going to perform the comet assay for detecting DNA Damage in mussels {green shells)..
Can anyone help me how much time is appropriate for the electrophoresis that is for how long the current should be applied and appropriate voltage and should we have to put our electrophoresis unit under yellow light to avoid UV Damage of DNA..As we have to carry out all work under UV light.
I will be really thankful if anybody solve my problems regarding comet assay.
thanks