Recent replies to "Indo-1 calcium imaging" Recent replies to "Indo-1 calcium imaging" http://network.nature.com/forums/natureprotocols/1132 en-us 40 Reply from anonymous <p>Did you figure it out? I&#8217;m not sure, but the excitation spectrum of the molecule may change with pH. Also, if only 3 AM esters are cleaved, maybe it&#8217;s binding another metal ion, with different emission properties. Did you check your indo stock with a calibration series?</p> <p>Good luck!</p> Mon, 28 Apr 2008 22:53:53 -0000 http://network.nature.com/forums/natureprotocols/1132?page=1#reply-3879 anonymous http://network.nature.com/forums/natureprotocols/1132?page=1#reply-3879