Recent replies to "How to distinguish necrosis from apoptosis" http://network.nature.com/forums/natureprotocols/1104 en-us 40 <p>Thanks, Bronwen! I really appreciate the suggestion. Pain or not, I&#8217;m certainly open to trying H&#38;E to distinguish the two, perhaps coupled with staining for activated caspases or other apoptosis-associated proteins.</p> Sat, 15 Mar 2008 20:25:29 -0000 http://network.nature.com/forums/natureprotocols/1104?page=1#reply-3137 Rebecca Riggins http://network.nature.com/forums/natureprotocols/1104?page=1#reply-3137 <p>When we were looking at cell death in mouse liver (induced by anti-Fas), we found that the only reliable method to distinguish apoptotic from necrotic cells was using morphological criteria (H&#38;E staining). This is a pain, I know&#8230;</p> <p>At the time, most clinical publications were reporting results from <span class="caps">TUNEL</span> staining with supporting data from immunohistochemistry for p53, Bcl-2 and/or Bax.<br />I suppose an alternative now might be immunohistochemistry of a favourite activated caspase&#8230;</p> Mon, 10 Mar 2008 15:43:56 -0000 http://network.nature.com/forums/natureprotocols/1104?page=1#reply-3045 Bronwen Dekker http://network.nature.com/forums/natureprotocols/1104?page=1#reply-3045