Recent replies to "hydrogen peroxide detection" http://network.nature.com/forums/natureprotocols/109 en-us 40 <p>I believe <span class="caps">NBT</span> isn&#8217;t very sensitive and has a certain threshold when the cell becomes completely positive. At least, that&#8217;s what we see in blood-cells. So it&#8217;s a black/white screening and not always points out that there isn&#8217;t any <span class="caps">H2O2</span> production if the cell is negative.</p> Wed, 01 Aug 2007 09:57:26 -0000 http://network.nature.com/forums/natureprotocols/109?page=1#reply-730 Vincent Groenewold http://network.nature.com/forums/natureprotocols/109?page=1#reply-730 <p>Hi, for measuring the peroxide with <span class="caps">DCF</span> you should use very low concentration of the dye.Also, you can use some recombinant fluorescent proteins to do so.See the paper published in Nature Methods(www.nature.com/nmeth/journal/v3/n4/abs/nmeth866.html)</p> Wed, 09 May 2007 16:35:15 -0000 http://network.nature.com/forums/natureprotocols/109?page=1#reply-265 Dinesh Kumar Singh http://network.nature.com/forums/natureprotocols/109?page=1#reply-265