how to calculate the amino groups
taqi ahmed khan
Monday, 21 April 2008 16:03 UTC
i am working on the topic of glycation of proteins.i have done the TNBS method for determining the amino groups present.i have made a standard plot by taking glcine.but i am now confused how to calculate the amino groups of my modified proteins
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Replies
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Hello This paper mite be useful in doing the TNBS assay. Analytical Biochemistry 14, 328-336 (1996)
“determination of Free Amino groupd in proteins By TNBS”.
Let me know if you want the soft copy of the paper.Sudha
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