Recent replies to "glycation of protein"

Reply from suji george

suji george — Tue, 08 Apr 2008 16:17:50 -0000

Hi,

The standard for fructosamine assay(NBT) is deoxymorphilino fructose, which is commercially available(Sigma).

Dr. Suji George

Reply from suji george

suji george — Mon, 07 Apr 2008 09:24:31 -0000

Hi,

1)Amadori product can be estimated by NBT assay, Amadori product has reducing property and it reduces dye NBT to blue colored formazan blue (Fructodamine assay).

2) Amadori can also be estimated by periodate oxidation.

2) Advanced glycation end product can be estimated by characteristic flourescence at 370ex/440 em and 335ex/385 em

Hope so this helps

If you need detailed protocol kindly email me at sujigeorge@mail.com

Reply from Cameron M

Cameron M — Thu, 03 Apr 2008 15:10:32 -0000

You could contract out MS Services, Protea Biosciences offers MS services. www.proteabio.com

Reply from taqi ahmed khan

taqi ahmed khan — Wed, 27 Feb 2008 09:25:27 -0000

i know that i can do the mass spectrometry.but i want the basic proocols not that techniques.because i dont have mass spectromete in my department.

Reply from Swastik Phulera

Swastik Phulera — Tue, 26 Feb 2008 06:01:01 -0000

do mass spectrophotometry and if you find a diffrence in molecular weights corrosponding to your hypothesis, what you say is correct