Variation of microinjected volume can make interpretation of Morpholino dose/response difficult in embryos. Here is an elegant solution: see the
supporting online material
for the methods for controlling Morpholino microinjected dose per embryo.
Riedel-Kruse IH, Muller C, Oates AC.
Synchrony dynamics during initiation, failure, and rescue of the segmentation clock.
Science. 2007 Sep 28;317(5846):1911-5. Epub 2007 Aug 16.