Recent replies to "Vivo-Morpholinos for adult animals"

Reply from Brian Schyth

Brian Schyth — Wed, 13 Feb 2008 21:38:18 -0000

Great – thanks for all the info. We will consider it!
Brian

Reply from Jon Moulton

Jon Moulton — Wed, 13 Feb 2008 17:44:29 -0000

Hi Brian,

There’s no publication yet and our only data are some images from a collaborator, which we can’t post since he might publish. The oligos were indeed injected into the blood of the fish, and had the expected phenotypic effect.

For prices, combine custom Morpholino with a 3’-Vivo-Porter: http://www.gene-tools.com/node/15

They are considerably more expensive than a bare oligo, but less than double the price. We do a multi-step synthesis on the oligo while its still bound to its synthesis resin, then cleave the modified oligo from the resin while deprotecting the bases, then go through some additional purification steps and dissolve the oligo in physiological saline. Vivo-Porter is shipped sterile in an ampule with a pierceable septum.

The first publication is still a few months off. When it appears, I’ll post the citation here in the Morpholino group.

Reply from Brian Schyth

Brian Schyth — Tue, 12 Feb 2008 13:47:31 -0000

Hi Jon,
Sounds good – I looked at the GeneTools homepage but did not see any data or references for zebrafish. You mention the use of the in vivo morpholinoes in zebrafish – could you tell me how these were applied to the fish (in the blood?) and how it worked in this model. I could imagine to use it as miRNA blocker or as an alternative to siRNAs – but I guess they are expensive?
Brian