Gene mapping - linkage and association: topic
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Genetic Map Distances
Lorna Houlihan
Friday, 20 July 2007 13:39 UTC
Hello,
I would like to know how I can convert Marshfield map positions to DeCode map positions, please? My data is in DeCode postitions and I would like to add in data which I have Marshfield co-ordinates. There are no corresponding DeCode postion information for these markers on the UCSC genome browser.
Hopefully someone can point me in the right direction, please?
Thanks,
Lorna
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Replies
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I found such data on the UCSC mysql server (the data is here , but you’ll need to known SQL or ask someone).
Hope it helps.
Pierre
mysql—user=genome—host=genome-mysql.cse.ucsc.edu -A -Dhg18
mysql> select name,marshfieldChrom,marshfieldPos,decodeChrom,decodePos from stsMap where marshfieldPos>0 or decodePos>0 limit 10\G
+++++++++++++ 1 row
name: AFM280WE5
marshfieldChrom: chr1
marshfieldPos: 4.22
decodeChrom: chr1
decodePos: 4
+++++++++++++ 2 row
name: AFM344WE9
marshfieldChrom: chr1
marshfieldPos: 8.85
decodeChrom: chr1
decodePos: 6.43
+++++++++++++ 3 row
name: AFM123XC3
marshfieldChrom: chr1
marshfieldPos: 10.78
decodeChrom: chr1
decodePos: 6.43
+++++++++++++ 4 row
name: AFMA203YC1
marshfieldChrom: chr1
marshfieldPos: 10.78
decodeChrom: chr1
decodePos: 7.09
+++++++++++++ 5 row
name: GATA68D01
marshfieldChrom: chr1
marshfieldPos: 10.78
decodeChrom: chr1
decodePos: 8.06
+++++++++++++ 6 row
name: AFMB355ZH5
marshfieldChrom: chr1
marshfieldPos: 11.87
decodeChrom: 0
decodePos: 0
+++++++++++++ 7 row
name: AFMA131YA5
marshfieldChrom: chr1
marshfieldPos: 11.87
decodeChrom: chr1
decodePos: 10.11
+++++++++++++ 8 row
name: AFMA052WG1
marshfieldChrom: chr1
marshfieldPos: 14.04
decodeChrom: chr1
decodePos: 11.37
+++++++++++++ 9 row
name: AFM254WB9
marshfieldChrom: chr1
marshfieldPos: 14.59
decodeChrom: 0
decodePos: 0
+++++++++++++ 10 row
name: AFMB039ZG9
marshfieldChrom: chr1
marshfieldPos: 14.04
decodeChrom: chr1
decodePos: 12
10 rows in set (0,31 sec) -
BTW,
you can also download this table from http://hgdownload.cse.ucsc.edu/goldenPath/hg18/database/stsMap.txt.gzPierre
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Thank you. However, it looks like some of my microsatellite markers do not have any DeCODE co-ordinates. Do you know if there is a way to extrapolate Marshfield co-ordinates to DeCODE co-ordinates?
Many thanks,
Lorna -
If you have flanking markers which are on the DeCode Map, you could use those to translate the DeCode map onto the Marshfield map. It won’t be very accurate doing it this way, but I guess it’s better than nothing.
So if you data looks like the following:
Marker DeCode Map Marshfield Map
Marker1 5 7
Marker2 5.5 7.4
Marker3 7.9
Marker4 6 8.3Calculate the Decode units per Marshfield unit for each marker and then use a mean of that value multiplied by the Marshfield position will give you an approximate location for your marker. Does that make sense?
Cheers,Claire
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Thanks Claire. It does make sense. Actually, I ended up using a program called Cartographer and for in-house markers, I used linear interpolation as done by DeCODE to estimate the DeCODE cM genetic distance.
Thanks for your help, Lorna
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